Anionic metabolite profiling by capillary electrophoresis-mass spectrometry using a noncovalent polymeric coating. Orange juice and wine as case studies.
Identifieur interne : 000336 ( Main/Exploration ); précédent : 000335; suivant : 000337Anionic metabolite profiling by capillary electrophoresis-mass spectrometry using a noncovalent polymeric coating. Orange juice and wine as case studies.
Auteurs : Tanize Acunha [Brésil] ; Carolina Sim [Espagne] ; Clara Ibá Ez [Espagne] ; Alberto Gallardo [Brésil] ; Alejandro Cifuentes [Espagne]Source :
- Journal of chromatography. A [ 1873-3778 ] ; 2016.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Anions, Polymers.
- analysis : Fruit and Vegetable Juices, Wine.
- chemistry : Citrus sinensis.
- methods : Food Analysis, Metabolomics.
- Electrophoresis, Capillary, Mass Spectrometry.
Abstract
In several metabolomic studies, it has already been demonstrated that capillary electrophoresis hyphenated to mass spectrometry (CE-MS) can detect an important group of highly polar and ionized metabolites that are overseen by techniques such as NMR, LC-MS and GC-MS, providing complementary information. In this work, we present a strategy for anionic metabolite profiling by CE-MS using a cationic capillary coating. The polymer, abbreviated as PTH, is composed of a poly-(N,N,N',N'-tetraethyldiethylenetriamine, N-(2-hydroxypropyl) methacrylamide, TEDETAMA-co-HPMA (50:50) copolymer. A CE-MS method based on PTH-coating was optimized for the analysis of a group of 16 standard anionic metabolites. Separation was achieved within 12min, with high separation efficiency (up to 92,000 theoretical plates per meter), and good repeatability, namely, relative standard deviation values for migration times and peak areas were below 0.2 and 2.1%, respectively. The optimized method allowed the detection of 87 metabolites in orange juice and 142 metabolites in red wine, demonstrating the good possibilities of this strategy for metabolomic applications.
DOI: 10.1016/j.chroma.2015.08.001
PubMed: 26296988
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000086
- to stream PubMed, to step Curation: 000086
- to stream PubMed, to step Checkpoint: 000086
- to stream Ncbi, to step Merge: 001C77
- to stream Ncbi, to step Curation: 001C77
- to stream Ncbi, to step Checkpoint: 001C77
- to stream Main, to step Merge: 000336
- to stream Main, to step Curation: 000336
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Anionic metabolite profiling by capillary electrophoresis-mass spectrometry using a noncovalent polymeric coating. Orange juice and wine as case studies.</title><author><name sortKey="Acunha, Tanize" sort="Acunha, Tanize" uniqKey="Acunha T" first="Tanize" last="Acunha">Tanize Acunha</name><affiliation wicri:level="2"><nlm:affiliation>Laboratory of Foodomics, CIAL, CSIC, Nicolas Cabrera 9, 28049 Madrid, Spain; CAPES Foundation, Ministry of Education of Brazil, 70040-020 Brasília, DF, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Laboratory of Foodomics, CIAL, CSIC, Nicolas Cabrera 9, 28049 Madrid, Spain; CAPES Foundation, Ministry of Education of Brazil, 70040-020 Brasília, DF</wicri:regionArea><placeName><region type="state">District fédéral (Brésil)</region></placeName></affiliation></author><author><name sortKey="Sim, Carolina" sort="Sim, Carolina" uniqKey="Sim C" first="Carolina" last="Sim">Carolina Sim</name><affiliation wicri:level="2"><nlm:affiliation>Laboratory of Foodomics, CIAL, CSIC, Nicolas Cabrera 9, 28049 Madrid, Spain. Electronic address: c.simo@csic.es.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Laboratory of Foodomics, CIAL, CSIC, Nicolas Cabrera 9, 28049 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Iba Ez, Clara" sort="Iba Ez, Clara" uniqKey="Iba Ez C" first="Clara" last="Ibá Ez">Clara Ibá Ez</name><affiliation wicri:level="2"><nlm:affiliation>Laboratory of Foodomics, CIAL, CSIC, Nicolas Cabrera 9, 28049 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Laboratory of Foodomics, CIAL, CSIC, Nicolas Cabrera 9, 28049 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Gallardo, Alberto" sort="Gallardo, Alberto" uniqKey="Gallardo A" first="Alberto" last="Gallardo">Alberto Gallardo</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Ciencia y Tecnología de Polímeros, ICTP-CSIC, Juan de la Cierva 3, 28006 Madrid, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Instituto de Ciencia y Tecnología de Polímeros, ICTP-CSIC, Juan de la Cierva 3, 28006 Madrid</wicri:regionArea><wicri:noRegion>28006 Madrid</wicri:noRegion></affiliation></author><author><name sortKey="Cifuentes, Alejandro" sort="Cifuentes, Alejandro" uniqKey="Cifuentes A" first="Alejandro" last="Cifuentes">Alejandro Cifuentes</name><affiliation wicri:level="2"><nlm:affiliation>Laboratory of Foodomics, CIAL, CSIC, Nicolas Cabrera 9, 28049 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Laboratory of Foodomics, CIAL, CSIC, Nicolas Cabrera 9, 28049 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:26296988</idno><idno type="pmid">26296988</idno><idno type="doi">10.1016/j.chroma.2015.08.001</idno><idno type="wicri:Area/PubMed/Corpus">000086</idno><idno type="wicri:Area/PubMed/Curation">000086</idno><idno type="wicri:Area/PubMed/Checkpoint">000086</idno><idno type="wicri:Area/Ncbi/Merge">001C77</idno><idno type="wicri:Area/Ncbi/Curation">001C77</idno><idno type="wicri:Area/Ncbi/Checkpoint">001C77</idno><idno type="wicri:Area/Main/Merge">000336</idno><idno type="wicri:Area/Main/Curation">000336</idno><idno type="wicri:Area/Main/Exploration">000336</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Anionic metabolite profiling by capillary electrophoresis-mass spectrometry using a noncovalent polymeric coating. Orange juice and wine as case studies.</title><author><name sortKey="Acunha, Tanize" sort="Acunha, Tanize" uniqKey="Acunha T" first="Tanize" last="Acunha">Tanize Acunha</name><affiliation wicri:level="2"><nlm:affiliation>Laboratory of Foodomics, CIAL, CSIC, Nicolas Cabrera 9, 28049 Madrid, Spain; CAPES Foundation, Ministry of Education of Brazil, 70040-020 Brasília, DF, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Laboratory of Foodomics, CIAL, CSIC, Nicolas Cabrera 9, 28049 Madrid, Spain; CAPES Foundation, Ministry of Education of Brazil, 70040-020 Brasília, DF</wicri:regionArea><placeName><region type="state">District fédéral (Brésil)</region></placeName></affiliation></author><author><name sortKey="Sim, Carolina" sort="Sim, Carolina" uniqKey="Sim C" first="Carolina" last="Sim">Carolina Sim</name><affiliation wicri:level="2"><nlm:affiliation>Laboratory of Foodomics, CIAL, CSIC, Nicolas Cabrera 9, 28049 Madrid, Spain. Electronic address: c.simo@csic.es.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Laboratory of Foodomics, CIAL, CSIC, Nicolas Cabrera 9, 28049 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Iba Ez, Clara" sort="Iba Ez, Clara" uniqKey="Iba Ez C" first="Clara" last="Ibá Ez">Clara Ibá Ez</name><affiliation wicri:level="2"><nlm:affiliation>Laboratory of Foodomics, CIAL, CSIC, Nicolas Cabrera 9, 28049 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Laboratory of Foodomics, CIAL, CSIC, Nicolas Cabrera 9, 28049 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Gallardo, Alberto" sort="Gallardo, Alberto" uniqKey="Gallardo A" first="Alberto" last="Gallardo">Alberto Gallardo</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Ciencia y Tecnología de Polímeros, ICTP-CSIC, Juan de la Cierva 3, 28006 Madrid, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Instituto de Ciencia y Tecnología de Polímeros, ICTP-CSIC, Juan de la Cierva 3, 28006 Madrid</wicri:regionArea><wicri:noRegion>28006 Madrid</wicri:noRegion></affiliation></author><author><name sortKey="Cifuentes, Alejandro" sort="Cifuentes, Alejandro" uniqKey="Cifuentes A" first="Alejandro" last="Cifuentes">Alejandro Cifuentes</name><affiliation wicri:level="2"><nlm:affiliation>Laboratory of Foodomics, CIAL, CSIC, Nicolas Cabrera 9, 28049 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Laboratory of Foodomics, CIAL, CSIC, Nicolas Cabrera 9, 28049 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author></analytic><series><title level="j">Journal of chromatography. A</title><idno type="eISSN">1873-3778</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anions (chemistry)</term><term>Citrus sinensis (chemistry)</term><term>Electrophoresis, Capillary</term><term>Food Analysis (methods)</term><term>Fruit and Vegetable Juices (analysis)</term><term>Mass Spectrometry</term><term>Metabolomics (methods)</term><term>Polymers (chemistry)</term><term>Wine (analysis)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Anions</term><term>Polymers</term></keywords><keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Fruit and Vegetable Juices</term><term>Wine</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus sinensis</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Food Analysis</term><term>Metabolomics</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Electrophoresis, Capillary</term><term>Mass Spectrometry</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In several metabolomic studies, it has already been demonstrated that capillary electrophoresis hyphenated to mass spectrometry (CE-MS) can detect an important group of highly polar and ionized metabolites that are overseen by techniques such as NMR, LC-MS and GC-MS, providing complementary information. In this work, we present a strategy for anionic metabolite profiling by CE-MS using a cationic capillary coating. The polymer, abbreviated as PTH, is composed of a poly-(N,N,N',N'-tetraethyldiethylenetriamine, N-(2-hydroxypropyl) methacrylamide, TEDETAMA-co-HPMA (50:50) copolymer. A CE-MS method based on PTH-coating was optimized for the analysis of a group of 16 standard anionic metabolites. Separation was achieved within 12min, with high separation efficiency (up to 92,000 theoretical plates per meter), and good repeatability, namely, relative standard deviation values for migration times and peak areas were below 0.2 and 2.1%, respectively. The optimized method allowed the detection of 87 metabolites in orange juice and 142 metabolites in red wine, demonstrating the good possibilities of this strategy for metabolomic applications.</div></front></TEI><affiliations><list><country><li>Brésil</li><li>Espagne</li></country><region><li>Communauté de Madrid</li><li>District fédéral (Brésil)</li></region></list><tree><country name="Brésil"><region name="District fédéral (Brésil)"><name sortKey="Acunha, Tanize" sort="Acunha, Tanize" uniqKey="Acunha T" first="Tanize" last="Acunha">Tanize Acunha</name></region><name sortKey="Gallardo, Alberto" sort="Gallardo, Alberto" uniqKey="Gallardo A" first="Alberto" last="Gallardo">Alberto Gallardo</name></country><country name="Espagne"><region name="Communauté de Madrid"><name sortKey="Sim, Carolina" sort="Sim, Carolina" uniqKey="Sim C" first="Carolina" last="Sim">Carolina Sim</name></region><name sortKey="Cifuentes, Alejandro" sort="Cifuentes, Alejandro" uniqKey="Cifuentes A" first="Alejandro" last="Cifuentes">Alejandro Cifuentes</name><name sortKey="Iba Ez, Clara" sort="Iba Ez, Clara" uniqKey="Iba Ez C" first="Clara" last="Ibá Ez">Clara Ibá Ez</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000336 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000336 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:26296988
|texte= Anionic metabolite profiling by capillary electrophoresis-mass spectrometry using a noncovalent polymeric coating. Orange juice and wine as case studies.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:26296988" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a OrangerV1
| This area was generated with Dilib version V0.6.25. |  |